猪皮肤中干细胞具有多向分化潜能

摘要：自动物体内获得的细胞可以做为研究发育、分化及核移植等方面的细胞模型。该研究自猪皮肤中，分离干细胞并检测其特性。该类细胞（PSOS）在体外呈球形克隆生长，表达神经前体标识分子－nestin，及Oct4及Stat3等多向潜能性相关的基因。且这种细胞在体外活跃增殖，长期培养后仍能保持正常的表型。在条件培养基中培养时，可产生具有神经元及星形胶质细胞特性的细胞。在含10％胎牛血清的培养基中培养时，细胞可以分化成类脂肪细胞。克隆性分析表明该细胞显示克隆性生长能力。自单个干细胞而来的细胞克隆在不同条件诱导下可以形成神经元样、胶质样及脂肪样细胞。该研究首次报道了自非啮齿类动物获得皮肤源性干细胞。

Stem cells with multilineage potential derived from porcine skin

Abstract：Stem cells from farm animals are valuable cell models for the study of development, differentiation, and are potential efficient donors for nuclear transfer. Here we report the isolation and characterization of stem cells from porcine skin. These porcine skin-originated sphere (PSOS) cells expressed the neural progenitor marker, nestin, as well as genes that are critical for pluripotency such as Oct4 and Stat3. The PSOS cells proliferated actively in vitro and retained normal karyotype after long-term culture. When cultured in defined medium, they generated cells with characteristics of neurons and astrocytes. A subpopulation of cells differentiated into adipocyte-like cells when cultured in 10% fetal bovine serum. Clonal study demonstrated that PSOS exhibited clonal-generating capability. Clonal populations from individual stem cells could form neuron-, astrocyte-, and adipocyte-like cells upon inducted differentiation. Our findings represent the first report of skin-originated stem cells isolated from non-rodent animals.

－Biochemical and Biophysical Research Communications  2004, 316：651-658