目的：牙周膜是由多种细胞组成的纤维结缔组织，其中包括牙周膜成纤维细胞。目前尚不清楚牙周膜成纤维细胞是否具有分化为其他类型细胞的潜能。方案：利用向Clawn小型猪牙周膜成纤维细胞中转染人端粒酶逆转录酶的方法建立细胞系并鉴定。结果：建立了TesPDL1-4四种牙周膜成纤维细胞系，具有成纤维样形态，表达alpha1前胶原蛋白、骨桥蛋白、periostin、碱性磷酸酶mRNA。在特定培养条件下，TesPDL3系细胞表达CD31、血管内皮细胞钙黏蛋白、血管性血友病因子、骨钙素，并形成细胞外矿化结节。结论：建立的TesPDL3细胞系在培养中表达成纤维细胞、血管内皮细胞和成骨细胞的若干表型。

Arch Oral Biol. 2007 May 31; [Epub ahead of print]

Establishment of cell lines that exhibit pluripotency from miniature swine periodontal ligaments.

Ibi M, Ishisaki A, Yamamoto M, Wada S, Kozakai T, Nakashima A, Iida J, Takao S, Izumi Y, Yokoyama A, Tamura M.

Department of Oral Biochemistry and Molecular Biology, Graduate School of Dental Medicine, Hokkaido University, North 13, West 7, Sapporo 060-8586, Japan; Department of Oral Functional Science, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.

OBJECTIVE: The periodontal ligament (PDL) is a fibrous connective tissue composed of heterogeneous cell types, including PDL fibroblasts. It is not clear whether cells within the PDL fibroblast population retain the potency to differentiate into other cell types. DESIGN: In the present study, clonal cell lines, derived from Clawn miniature swine PDLs, were established by gene transfection for a human telomerase reverse transcriptase, and characterized. RESULTS: These cell lines, denoted TesPDL1-4, had PDL fibroblasts that showed fibroblastic morphology and expressed procollagen alpha1(I), osteopontin, periostin and alkaline phosphatase mRNA. Under the specific culture conditions, TesPDL3 cells also have the ability to express CD31, vascular endothelial cadherin, von Willebrand factor, osteocalcin, and to form extracellular mineralized nodules. CONCLUSIONS: Our data indicate that TesPDL3 cells have unique properties of expressing several phenotype of fibroblasts, vascular endothelial cells and osteoblasts in cultures.

Establishment of cell lines that exhibit pluripotency from miniature swine periodontal ligaments.pdf