Biochemical and Biophysical Research Communications 361 (2007) 883–889
Isolation and identification of mesenchymal stem cells from human lipoma tissue

Lipoma is a benign neoplasm of normal fat cells that appears as a soft, movable swelling, often with a slight yellowish coloration. Human mesenchymal stem cells (MSCs) that have been isolated from bone marrow, blood, and other adult tissues including adipose tissue have the potential to be useful candidates for therapy. No literature had reported about stem cells from lipoma tissue. Here, a new cell culture method is described and utilized to greatly accelerate the growth rate and prolong the lifespan of lipoma-derived MSCs. Cells produced in early cultures display characteristics similar to those previously reported for multipotential stem cells, including a high frequency of anchorage-independent growth in soft agar and a lack of gap junctional intercellular communication in cell types with serpiginous morphology. These cells can differentiate into adipocytes, osteoblasts, and chondrocytes after induction. In conclusion, lipomaderived stem cells possessing the characteristics of MSCs are described for the first time.

我们可以从骨髓、血液、脂肪等组织中分离到人间充质细胞。脂肪瘤是一种良性肿瘤，近来，来自台湾的学者首次介绍一种从脂肪瘤中分离培养间充质干细胞（LDMSC）的方法，能够得到增殖率高，平均寿命长的间充质细胞。这些细胞表现出干细胞的特性，包括不依赖滋养层，细胞间缺乏缝隙连接等，经诱导可以分化为脂肪细胞，成骨细胞和软骨细胞。因此脂肪瘤有望成为间充质干细胞的新来源，但是，LDMSC的寿命有限，多数细胞到第9代时开始死亡。而且，亦不能排除LDMSC恶变的可能。