Biochem Biophys Res Commun. 2008 Apr 4;368(2):329-35. Epub 2008 Jan 28. Links

Characterisation of human dental stem cells and buccal mucosa fibroblasts.

Lindroos B, Mäenpää K, Ylikomi T, Oja H, Suuronen R, Miettinen S.

REGEA, Institute for Regenerative Medicine, University of Tampere and Tampere University Hospital, Biokatu 12, 33520 Tampere, Finland. bettina.lindroos@regea.fi

Human craniofacial stem cells are recently discovered sources of putative mesenchymal stem cells that hold great promise for autogenic or allogenic cell therapy and tissue engineering.、   Prior to employing these cells in clinical applications, they must be thoroughly investigated and characterized. In this study, the surface marker expression was investigated on dental pulp stem cells (DPSCs), dental follicle cells (DFCs), periodontal ligament stem cells (PDLSCs), and buccal mucosa fibroblasts (BMFs) utilising surface markers for flow cytometry. The osteogenic potential was also examined by bone-associated markers alkaline phosphatase, Runx2, collagen type I, osteocalcin, and osteopontin. The results from our study demonstrate that the dental cell sources exhibit comparable surface marker and bone-associated marker profiles parallel to those of other mesenchymal stem cell sources, yet distinct from the buccal mucosa fibroblasts. Our data support evidence towards clinical applicability of dental stem cells in hard tissue regeneration.

PMID: 18230338 [PubMed - in process]

人牙齿干细胞和颊粘膜成纤维细胞的特征

    最近的研究指出，人类颅面部组织来源的干细胞起源于间充质干细胞，非常有希望应用于自体或异体细胞治疗和组织工程。临床上应用此类细胞之前必须充分研究了解其特性。在本研究中，运用流式细胞计数技术对牙髓干细胞、牙囊细胞、牙周膜干细胞和颊粘膜成纤维细胞的表面标记物表达进行了研究。并对骨相关标志物:碱性磷酸酶、Runx2、Ⅰ型胶原、骨钙素、骨桥蛋白的表达进行检测，观察其成骨能力。我们的研究结果表明，牙齿来源的细胞其表面标记物和骨相关标志物与其他间充质干细胞来源的细胞相似，但与颊粘膜成纤维细胞不同。我们的研究为临床应用牙齿干细胞进行硬组织再生提供了依据。