Human adipose–derived mesenchymal stem cells (hAD-MSCs) were cultured in growth medium of low
calcium concentration to which antioxidants had been added. Large numbers of hAD-MSCs could be
obtained within 2 weeks. Scaffolding was made with 3 commonly used biomaterials. Gelatin sponges and
polyglycolic acid meshes were cut into small pieces and put into the scaffold pocket, which was made of
polypropylene mesh and measured 1.51.00.5 cm3. Immune-deficient mice were divided into 3 groups.
In Group I, only hAD-MSCs were injected. In Group II, the scaffold alone was implanted and harvested
after 2 months in vivo. In Group III, scaffolds were cultured with hAD-MSCs in adipogenic medium for 2
weeks before implantation, and 2 implanted scaffolds were harvested after 2, 4, and 6 months in vivo. All
of the successfully harvested scaffolds were filled with newly formed adipose tissue and had retained their
predefined shape and dimensions. New blood vessels had also grown into the newly formed adipose tissue.
Adipose tissue of specific shape and 3 dimensions was regenerated in vivo using tissue engineering of
hAD-MSCs and scaffolding made with 3 common biomaterials.

人脂肪来源的间充质干细胞构建工程化脂肪组织　　

用低钙并含有抗氧化剂的生长培养基培养人脂肪来源的间充质干细胞（hAD-MSCs）。在2周内获得大量的hAD-MSCs。用三种普通的生物材料做为支架。将明胶海绵和聚乙醇酸切成小块并放入支架囊中，这种支架囊是由1.5×1.0×0.5cm³的聚丙烯构成。将免疫缺陷小鼠分成三组。第一组，只注射hAD-MSCs,第二组，只有支架材料，并两个月后取材，第三组，hAD-MSCs复合支架并在脂肪培养基中培养2周然后移植两种移植的支架分别在移入体内2，4，6月后取材。所有的成功获取的支架都含有新形成的脂肪组织并保持固定的形状和尺寸。并且有新生血管长入新形成的脂肪组织。用hAD-MSCs复合3种通用的生物材料可以在体内形成特定形状及三维的脂肪组织。